Journal: Balkan Medical Journal
Article Title: Comparison of Different ROS1 Immunohistochemistry Clones and Consistency with Fluorescence In Situ Hybridization Results in Non-Small Cell Lung Carcinoma
doi: 10.4274/balkanmedj.galenos.2023.2022-12-88
Figure Lengend Snippet: Representative comparative samples of the hematoxylin and eosin (H&E), ROS1 IHC (SP384 and D4D6), and ROS1 FISH images. (a)-(d) represent samples 6, 7, 14, and 15, respectively. (a) [1 +] weak staining with D4D6 IHC and [2 +] moderate staining with SP384 IHC in isolated 3' (42%) pattern ROS1 FISH-positive. (b) [2 +] moderate staining with D4D6 IHC and [3 +] strong staining with SP384 IHC in isolated 3′ (67%) pattern ROS1 FISH-positive. (c) [2 +] moderate staining with D4D6 IHC and [3 +] strong staining with SP384 IHC in break apart (94%) pattern ROS1 FISH-positive. (d) [0] negative staining with D4D6 IHC and [3+] strong staining with SP384 IHC in ROS1 FISH-negative. H&E and IHC digital images are at 200x, and FISH digital images are at 600x magnification.
Article Snippet: The AmoyDX ROS1 Gene Fusion Detection Kit (Amoy Diagnostics Co., Ltd, Xiamen, China) is a CE-IVD-labeled RT-PCR to detect the 14 most common ROS1 fusions.
Techniques: Staining, Isolation, Negative Staining